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Curriculum Vitae

BroussardCV

 

Gregory W. Broussard

The Pennsylvania State University

Biochemistry and Molecular Biology

122B South Frear

University Park, PA 16802

Wk: (814) 863-0502

gwb13@psu.edu

Employment

 

Lecturer                                                                                 2014-present

The Pennsylvania State University

Biochemistry and Molecular Biology

 

Research Associate                                                                2011-2014

University of Pittsburgh, Pittsburgh, PA

Department of Biological Sciences

Laboratory: Dr. Graham F. Hatfull

Education/Training

 

Postdoctoral Training                                                           2007-2011

University of Pittsburgh, Pittsburgh, PA

Department of Biological Sciences

Laboratory: Dr. Graham F. Hatfull

 

Ph.D. (Biology)                                                                      2001-2007

University of Louisiana, Lafayette, LA,

Department of Biology

Laboratory: Dr. Don G. Ennis

B.S. (Microbiology)                                                               1996-2001

University of Louisiana, Lafayette, LA

Department of Biology

Minors: Chemistry and Psychology

Course Teaching Experience

 

Courses Taught at Penn. State:

 

Medical Microbiology Laboratory (MICRB 422): Spring 2015

Laboratory demonstrating properties and classification of medically important microorganisms and techniques used in their identification.

Laboratory in Molecular Immunology (MICRB 447): Spring 2015

Laboratory in molecular techniques to assay antigens, antibodies and receptor sites.

Introductory Microbiology Laboratory (MICRB 202): Spring 2015

(Lab coordinator of seven sections)

Qualitative and quantitative techniques with regard to recognition of bacteria and their processes on a microscopic, colonial, and physiological basis.

Laboratory of General and Applied Microbiology (MICRB 421W): Fall 2014

Laboratory demonstrating fundamental techniques and principles of experimentation of general and applied microbiology.

Elementary Microbiology (MICRB 106): Fall 2014

Importance of microorganisms in health and disease, agriculture, and industry; descriptive course for students not planning advanced study in microbiology.

 

 

Courses Taught at Other Univesities: (During Graduate School)

 

Media Preparation, University of Louisiana: Fall 2002, Spring 2003, Spring 2004

Developed syllabus, lectured, wrote exams, graded exams, and instructed laboratory work. Course involved an in depth knowledge of the components of different media commonly used in a clinical microbiology laboratory setting. Media and solutions produced were used to supply concurrent microbiology courses.

Molecular and Cellular Engineering Laboratory, University of Louisiana: Fall 2003

Instructed eleven graduate students and one undergraduate. Developed syllabus, lectured, wrote exams, graded exams, and instructed laboratory work. Course involved molecular techniques, including: manipulation of DNA, transformation of bacteria, Southern and Western Blots, and protein purification, among others.

Individual Contact Teaching Experience

 

Phagehunter Program, University of Pittsburgh, 2007-2014

Participated in the phagehunter program, in the laboratory of Dr. Graham Hatfull, aimed at education in science focused on undergraduates and high school students. Oversaw journal club meetings and participated in general meetings.

Undergraduate Research Instruction, University of Pittsburgh, 2007-2014

Mentored ten undergraduates conducting individual research projects over the course of my post-doctoral and research associate career in Dr. Graham Hatfull’s lab. Projects included phage discovery, genome sequencing, and exploring the functional genomics of various aspects of phage biology. Within this group, individuals published results in scientific journals, entered graduate school and entered medical school.

University of Louisiana: 2001-2007

Mentored eleven undergraduates over the course of my graduate career on laboratory research concerning aspects of my dissertation. Undergraduates engaged in all aspects of the laboratory, including lab meetings, discussion of research goals, and problem solving strategies. Experiences involved aspects of bacteriology, ichthyology, pathology, immunology, molecular biology, and disease modeling.

Instructed five undergraduates in a research project that resulted in the publication of a journal article: Civilian household bioterrorism decontamination agents. Journal of Undergraduate Chemistry Research 4: 165-168.

Research Interests

Phage functional genomics; phage-host interactions; bacterial pathogens, especially M. tuberculosis and Vibrio spp.

Awards, Grants, Fellowships

 

NIH training grant in Molecular Microbial Persistence and Pathogenesis, 2008-2010

            Award included 2 yrs. postdoctoral stipend and travel to scientific meetings

 

Award for Oral Presentation, 2006

            Wind River Conference on Prokaryotic Biology

Award: Book, Gram Positive Pathogens, 2nd Ed., ASM Press

 

Three Ravin-Rogers travel award, 2003, 2004, 2006

Wind River Conference on Prokaryotic Biology

Total award: $800

 

AAAS/Science Program for Excellence in Science, 2006

Award included free membership to AAAS and free subscription to Science.

Ten UL Graduate Student Organization Travel Grants, 2001-2006

Grants awarded for travel to various meetings throughout graduate career

Total award: $2,400.00

Fourteen UL Graduate Student Organization Supply Grants, 2001-2006

Grants awarded for research supplies throughout graduate career

Total award: $2,240.00

ULL Fellowship, 2001-2005

Tuition and stipend during graduate school

Duties included two years of teaching responsibility and presentations at

departmental seminars

Award for Oral Presentation, 2005

Department of Biology Graduate Student Symposium

University of Louisiana

Professional Reviewer Activities

Associate Faculty Member, Faculty of 1000, 2012-2014

Manuscript Co-Reviewer, Tuberculosis, 2013

Manuscript Reviewer, Journal of Aquatic Animal Health, 2003

Invited/Visiting Scientist Activities

 

China Agricultural University, November 18th to December 2nd 2012

Collaborative work on recombineering in M. bovis at the College of Veterinary Medicine.

 

Professional Memberships

 

American Society for Microbiology, 2001-2014

The American Association for the Advancement of Science, 2006-2014

American Society for Microbiology, Allegheny Branch, 2008-2010, 2012-2014

International Society for Viruses of Microbes, 2012-2013

Phages for Human Application Group Europe, 2012

Aquatic Models Society, 2006-2008

American Society for Microbiology, South Central Branch, 2001-2007

American Society for Microbiology, Texas Branch, 2001-2003

GenBank Entries

Mycobacterium phage Acadian, complete genome, accession number JN699007
Publications

Peer-Reviewed Publications

  1. Pope, W.H, Broussard, G.W. (6th of 41 authors), et. al., 2013. Mycobacteriophages Rey, PegLeg and Bongo constitute the new Cluster M. Journal of Virology. (submitted)
  1. Pope, W.H., Jacobs-Sera, D., Best, A.A., Broussard, G.W., et al., 2013. Cluster J mycobacteriophages: Intron splicing in capsid and tail genes. PLOS One 8(7), e69273.
  1. Silva, J., Piuri, M., Broussard, G., Marinelli, L., Bastos, G., Hirata, R., Hatfull, G., Hiratta, M., 2013. Application of BRED technology to construct recombinant D29 reporter phage expressing EGFP. FEMS Microbiology Letters 344, 166-172.
  1. Broussard, G.W. and Hatfull, G.F., 2013. Evolution of genetic switch complexity. Bacteriophage 3(1), e24186.
  1. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F., 2013. Integration-dependent bacteriophage immunity provides insights into the evolution of genetic switches. Molecular Cell 49(2), 237-248.
  1. Jacobs-Sera, D., Marinelli, L., Bowman, C., Broussard, G., Guererro, C., Boyle, M., Petrova, Z., Dedrick, R., Pope, W., SEA-PHAGES PROGRAM; Modlin, R., Hendrix, R., Hatfull, G., 2012. On the nature of mycobacteriophage diversity and host preference. Virology 434, 187-201.
  1. Traag, B.A., Driks, A., Stragier, P., Bitter, W., Broussard, G., Hatfull, G., Chu, F., Adams, K.N., Ramakrishnan, L., Losick, R., 2010. Do mycobacteria produce endospores? PNAS 107, 878-881.
  1. Sampson, T., Broussard, G.W., Marinelli, L.J., Jacobs-Sera, D., Ray, M., Ko, C-C., Russel, D., Hendrix, R.W., Hatfull, G.F., 2009. Mycobacteriophages BPs, Angel and Halo: comparative genomics reveals a novel class of ultra-small mobile genetic elements. Microbiology 155, 2962-2977.
  1. Broussard, G.W., Norris, M.B., Schwindt, A.R., Fournie, J.W., Winn, R.N., Kent, M.L., Ennis, D.G., 2009. Chronic Mycobacterium marinum infection acts as a tumor promoter in Japanese medaka. Comparative Biochemistry and Physiology, Part C 149, 152-160.
  1. Broussard, G.W., and Ennis, D.G., 2007. Mycobacterium marinum produces long-term chronic infections in medaka: a new animal model for studying human tuberculosis. Comparative Biochemistry and Physiology, Part C 145, 45-54.
  1. Flaherty, D., Guidry, L.A., Guilbeau, K.M., Lewis, A.M., Welch, C.A., Trahan, A., Broussard, G.W., Antley, P., and Taylor, E.R. 2004. Civilian household bioterrorism decontamination agents. Journal of Undergraduate Chemistry Research 4: 165-168.

Dissertation

Broussard, G.W. 2007. Development and Application of Medaka and Mycobacterium marinum as an Infection Model for Human Tuberculosis. (Doctoral Dissertation) University of Louisiana, Lafayette, Louisiana.

 

Presentations

 

Invited Oral Presentations:

 

  1. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F., 2012. Integration-dependent bacteriophage immunity provides insights into the evolution of genetic switches. Presentation to the Student Body at the College of Veterinary Medicine, China Agricultural University.

 

  1. Broussard, G., 2012. Discovery and exploitation of mycobacteriophages. China Institute of Veterinary Drug Control, Center for Veterinary Drug Evaluation, MOA.

 

  1. Broussard, G.W., Oldfield, L., Villanueva, V., Lunt, B., Shine, E., Hatfull, G.F., 2012. Integration-dependent bacteriophage immunity provides insights into the evolution of genetic switches. Chinese Academy of Sciences.

 

Conference Oral Presentations:

  1. Broussard, G.W., Oldfield, L.M., Villanueva, V.M., Hatfull, G.H., 2012. A novel self-contained genetic switch. Pittsburgh Bacterial Meeting, Duquesne University.
  1. Broussard, G.W., Oldfield, L.M., Villanueva, V.M., Hatfull, G.H., 2011. A novel self-contained genetic switch. First Annual Postdoctoral Research Symposium, Dept. Bio. Sci., Univ. Pittsburgh.
  1. Broussard, G.W., Oldfield, L.M., Villanueva, V., Hatfull, G.F., 2010. Integration is a prerequisite for mycobacteriophage BPs lysogeny establishment: a novel regulatory mechanism for genetic networks. Pittsburgh Bacterial Meeting, Duquesne University.
  1. Mutoji, N.K., Wipple, C., Broussard, G.W., Guidry, L., Durling, L., Ennis, D.G., 2008. Exploration into potential modes of transmission of Mycobacterium marinum in Japanese medaka (Oryzias latipes). The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W., Zaunbrecher, L.K., Norris, M.B., Winn, R.N., Fournie, J.W., Schwindt, A.R., Kent, M.L., Ennis, D.G.*, 2008. Evidence for increased cancer risks with chronic mycobacterial infections. The Annual Meeting of the Wind River Conference on Prokaryotic Biology. (Presented by Ennis, D.G.)
  1. Broussard, G.W. and Ennis, D.G. 2006. Development of a Tuberculosis Model Using the Small Fish Japanese Medaka and Mycobacterium marinum: Application of See-Through Medaka. The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W. and Ennis, D.G. 2006. Mycobacterium marinum produces long-term chronic infections in medaka: a new animal model for studying human tuberculosis. American Society for Microbiology, South Central Branch Meeting.
  1. Broussard, G.W. and Ennis, D.G. 2005. Development of a tuberculosis model using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Sixth Annual Department of Biology Graduate Student Symposium, University of Louisiana.
  1. Ennis, D.G. and Broussard, G.W. Establishing medaka as a small fish model for Mycobacterium marinum infections. The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W. and Ennis, D.G. 2005. Development of a tuberculosis model using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Annual Eastern Fish Health Workshop.
  1. Ennis, D.G. and Broussard, G.W. Establishing medaka as a small fish model for Mycobacterium marinum infections. Aquatic Animal Models of Human Disease Conference.
  1. Broussard, G.W. and Ennis, D.G. 2004. Establishing medaka as a small fish model for Mycobacterium marinum Fifth Annual Department of Biology Graduate Student Symposium, University of Louisiana.
  1. Broussard, G.W. and Ennis, D.G. 2004. Roles of Mycobacterial DNA repair networks in fish pathogenesis. University of Louisiana, Biology Department Seminar.
  1. Broussard, G.W. and Ennis, D.G. 2004. Establishing medaka as a small fish model for Mycobacterium marinum The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W., Zaunbrecher, A., Durling, L., and Ennis, D.G. 2004. Establishing medaka as a small fish model for Mycobacterium marinum American Society for Microbiology, South Central Branch Meeting.
  1. Broussard, G.W. and Ennis, D.G. 2003. Using small fish models to detect mutagenesis induction by chronic inflammation. University of Louisiana, Biology Department Seminar.
  1. Ennis, D.G. and Broussard, G.W. Establishing medaka (Oryzias latipes) as a small fish model for Mycobacterium marinum infections. Aquatic Animal Models of Human Disease Conference.

Poster Presentations:

  1. Cathcart, A., Broussard, G.W., Hatfull, G.F., 2013. Characterization of highly conserved promoter-associated sequences in Cluster B mycobacteriophages. University of Pittsburgh, Science 2013 Meeting.
  1. Oldfield, L.M., Broussard, G.W., Villanueva, V.M., Hatfull, G.F., 2012. Control of the BPs lytic-lysogenic genetic switch by divergent promoters. University of Pittsburgh, Science 2012 Meeting.
  1. Oldfield, L.M., Broussard, G.W., Villanueva, V.M., Hatfull, G.F., 2012. Control of the BPs lytic-lysogenic genetic switch by divergent promoters. University of Pittsburgh, Department of Biological Sciences Retreat at PLE.
  1. Oldfield, L., Broussard, G., Villanueva, V., Hatfull, G., 2012. Gene expression of mycobacteriophage BPs. Viruses of Microbes Meeting.
  1. Villanueva V., Broussard, G., Oldfield L., Hatfull, G., 2012. Characterization of a novel phage repressor. Viruses of Microbes Meeting.
  1. Broussard, G., Oldfield, L., Villanueva, V., Shine, E., Lunt, B., Hatfull, G., 2012. A novel lysogenic genetic circuit. Viruses of Microbes Meeting.
  1. Villanueva, V.M., Broussard, G.W., Hatfull, G.F., 2011. The unusual immunity system of mycobacteriophage BPs. American Society for Microbiology General Meeting.
  1. Oldfield, L.M., Broussard, G.W., Hatfull, G.F., 2011. Activation of the mycobacteriophage BPs repressor by integration: a novel phage regulatory mechanism. American Society for Microbiology General Meeting.
  1. Schmidt, L., Broussard, G., Jacobs-Sera, D., Hatfull, G., 2011. Mycobacteriophage DaVinci reveals a genomic switch to a partitioning system for prophage integration. American Society for Microbiology General Meeting.
  1. Broussard, G.W., Oldfield, L.M., Villanueva, V., Sampson, T., Hatfull, G.F., 2010. Activation of the mycobacteriophage BPs repressor by integration: a novel phage regulatory mechanism. American Society for Microbiology General Meeting.
  1. Broussard, G.W., Bowman, C., Hatfull, G.F., 2009. Mycobacteriophage lsr2 genes: why have them? Pittsburgh Bacterial Meeting.
  1. Broussard, G.W., Bowman, C., Cresawn, S., and Hatfull, G.F., 2008. May I complement your Lsr2: Why do mycobacteriophage have Lsr2 homolgues? University of Pittsburgh’s Science 2008 Meeting.
  1. Broussard, G.W., Bowman, C., Cresawn, S., and Hatfull, G.F., 2008. May I complement your Lsr2: Why do mycobacteriophage have Lsr2 homolgues? University of Pittsburgh, Department of Biological Sciences Symposium.
  1. Broussard, G.W. and Hatfull, G.F., 2008. Investigation into the ability of mycobacteriophage lsr2 homologues to complement a lsr2 defect in Mycobacterium. American Society for Microbiology General Meeting.
  1. Mutoji, N.K., Broussard, G.W., Ennis, D.G., 2008. Biofilm formation and its effect on virulence of Mycobacterium marinum in medaka (Oryzias latipes). The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W. and Ennis, D.G., 2006. Mycobacterium marinum produces long-term chronic infections in medaka: a new animal model for studying human tuberculosis. American Society for Microbiology, South Central Branch Meeting.
  1. Mutoji, N.K., Broussard, G.W., Guidry, L., Durling, L., and Ennis. D.G., 2006. Natural modes of transmission of Mycobacterium marinum in medaka (Oryzias latipes). American Society for Microbiology, South Central Branch Meeting.
  1. Broussard, G.W. and Ennis, D.G., 2005. Development of a tuberculosis model using the small fish medaka (Oryzias latipes) and Mycobacterium marinum. Aquatic Animal Models of Human Disease Conference.
  1. Mutoji, N.K., Broussard, G.W., Guidry, L., Durling, L., and Ennis, D.G., 2005. Natural modes of transmission of Mycobacterium marinum in medaka (Oryzias latipes). Aquatic Animal Models of Human Disease Conference.
  1. Broussard, G.W., Brown, Y., and Ennis, D.G. 2003. Using the small fish model medaka in an effort to link chronic infection and mutagenesis. The Annual Meeting of the Wind River Conference on Prokaryotic Biology.
  1. Broussard, G.W., Granados, L.S., and Ennis, D.G. 2002. Studying mycobacterial infection using small fish models and marinum expressing Gfp. The Fourth International Symposium on Aquatic Animal Heath.
  1. Broussard, G.W., Granados, L.S., and Ennis, D.G. 2002. Studying mycobacterial infection using small fish models and marinum expressing Gfp. American Society for Microbiology, South Central Branch Meeting.
  1. Granados, L.S., Lemoine, F.J., Marriott, S.J., Higginbotham, R.H., Broussard, G.W., and Ennis, D.G. 2002. Effect of HTLV-1 Tax expression on spontaneous and UV-induced mutagenesis. American Society of Microbiology, South Central Branch Meeting.
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