Making Primer Stocks

Gregory Broussard

Revised: 8/1/07

(Please use, change and disseminate freely!)

1. When receiving a new lyophilized primer start by making a 100 µM stock.  Multiply the number of nmoles printed on the tube by 10.  This is the number of µl of TE to add to the tube to get a 100 µM stock.  Make this stock directly in the tube containing the primer.
2. Make a 10 µM working stock by making a 1:10 dilution of the 100 µM stock.  Use this for your PCR reactions